Gelite™ Safe DNA Gel Stain *10,000X DMSO Solution*
Catalogue Number: 17707-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Gel Stain |
| Shipping Condition: | Blue Ice |
| Unit(s): | 10 ml |
Description
Description: Biotin NTA (BNTA), a biotin-labeled nitrilotriacetic acid, is widely used to detect histidine-tagged proteins immobilized on nitrocellulose membranes with the detection limite of ~0.1 pmol. NTA Biotin is a bifunctional reagent that is used to detect histidine-tagged proteins immobilized. The nitrilotriacetic acid is used to chelate a Ni(II) ion at four of its six coordination sites. The remaining two sites are available for binding to a histidine tag. The NTA-polyHis-complex can be detected using standard enzyme-linked streptavidin methods, such as a streptavidin-horseradish peroxidase conjugate. Biotin NTA can be removed from the histidine-tagged protein at pH 4.8, allowing the blot to be reanalyzed with another probe. In combination with fluorescent avidin conjugates, this NTA biotin derivative can be used for detecting polyhistidine-containing biomolecules such as fusion proteins.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|12 months
17707 Protocol
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