Nucleosome Assembly 601 Sequence DNA, Biotinylated
Catalogue Number: 18-0005-EPC
| Manufacturer: | EpiCypher |
| Physical state: | Lyophilized |
| Type: | Nucleosome |
| Host Cell: | Synthetic DNA |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
Description
Description: Nucleosome Assembly 601 Sequence DNA is a 147 base-pair double-stranded DNA fragment that was identified by Lowary and Widom using the SELEX method [1]. The 601 Sequence DNA has high affinity for histone octamers and is useful for in vitro nucleosome assembly. The DNA contains a 5' biotin-TEG group.
Additional Text
Preparation
Lyophilized
Application Notes
Nucleosome Assembly 601 Sequence DNA is useful for assembly of nucleosomes using purified or recombinant histone octamers (EpiCypher 16-0001). See Luger et al. [2] for recommended nucleosome reconstitution protocol. The biotin group on the DNA allows for pull-down of the nucleosomes for nucleosome binding experiments or enzymatic assays.
Storage Note
Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -80°C
18-0005 Datasheet
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