EpiDyne® Remodeling Assay Substrate DNA ST601-GATC0
Catalogue Number: 18-4100-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 24 months |
| Physical state: | Lyophilized |
| Type: | Substrate |
| Host Cell: | Synthetic DNA|Human |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: EpiDyne® Remodeling Assay Substrate DNA ST601-GATC0 is a 217 base-pair double-stranded DNA fragment containing the 601 nucleosome positioning sequence [1], which has high affinity for histone octamers and is useful for nucleosome assembly. The DNA also includes a 3' acceptor sequence to accommodate the histone octamer subsequent to remodeling. This negative control does not contain DpnII restriction sites. When paired with the positive control DNA (Catalog No. 18-4101) these controls illustrate the migration range for the Restriction Enzyme Assay. See the EpiDyne Nucleosome Remodeling Assay Tech Note (Restriction Enzyme Assay) for more information: epicypher.com/resources/technical-notes
Additional Text
Protein Tag
Untagged
Application Notes
ST601-GATC0 DNA is useful as a negative control for restriction enzyme accessibility nucleosome remodeling assays using the EpiDyne Remodeling Assay Substrate. No DpnII restriction enzyme site is present, while the naturally occurring MfeI (AATTGG in bold, below) is present within the 601 sequence.
Storage Note
Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -80°C.
18-4100 Datasheet
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