EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1
Catalogue Number: 18-4101-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 24 months |
| Physical state: | Lyophilized |
| Type: | Substrate |
| Host Cell: | Synthetic DNA|Human |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1 is a 217 base-pair double-stranded DNA fragment. The sequence includes an upstream 6 bp linker, the 145 bp ST601 nucleosome positioning sequence, identified by Lowary and Widom [1], and a downstream 66 bp linker (hereby named 6-N-66 DNA). The DNA also includes a 3' acceptor sequence to accommodate the histone octamer subsequent to remodeling. This positive control has a DpnII restriction site within the 601 sequence. When paired with the negative control (EpiCypher 18-4100), these controls illustrate the migration range for the Restriction Enzyme Assay. For more information, see the EpiDyne Nucleosome Remodeling Assay Tech Note: epicypher.com/resources./technical-notes
Additional Text
Protein Tag
Untagged
Application Notes
ST601-GATC1 DNA is useful as a positive control for restriction enzyme accessibility nucleosome remodeling assays using the Biotinylated EpiDyne Remodeling Assay Substrate (EpiCypher 16-4101). A DpnII restriction enzyme site (GATC in red, below) and the naturally occurring MfeI restriction site (AATTGG in bold, below) are present within the 601 sequence.
Storage Note
Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -80°C.
18-4101 Datasheet
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