EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1
Catalogue Number: 18-4101-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 24 months |
| Physical state: | Lyophilized |
| Type: | Substrate |
| Host Cell: | Synthetic DNA|Human |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1 is a 217 base-pair double-stranded DNA fragment. The sequence includes an upstream 6 bp linker, the 145 bp ST601 nucleosome positioning sequence, identified by Lowary and Widom [1], and a downstream 66 bp linker (hereby named 6-N-66 DNA). The DNA also includes a 3' acceptor sequence to accommodate the histone octamer subsequent to remodeling. This positive control has a DpnII restriction site within the 601 sequence. When paired with the negative control (EpiCypher 18-4100), these controls illustrate the migration range for the Restriction Enzyme Assay. For more information, see the EpiDyne Nucleosome Remodeling Assay Tech Note: epicypher.com/resources./technical-notes
Additional Text
Protein Tag
Untagged
Application Notes
ST601-GATC1 DNA is useful as a positive control for restriction enzyme accessibility nucleosome remodeling assays using the Biotinylated EpiDyne Remodeling Assay Substrate (EpiCypher 16-4101). A DpnII restriction enzyme site (GATC in red, below) and the naturally occurring MfeI restriction site (AATTGG in bold, below) are present within the 601 sequence.
Preparation
Lyophilized
Storage Note
Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -80°C.
18-4101 Datasheet
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