Anti-RBPMS Antibody
Catalogue Number: 1832-RBPMS-PPS
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Purified mouse RGCs in culture that have been probed by FISH for Runx1(red), immunostained with an RGC marker RBPMS(green, cat. 1832-RBPMS), and DAPI(blue). (A) An section where two Runx1+ RGCs outlined with dashed box are shown enlarged below (B,C; cell soma outlined with dashed line). Photo courtesy Bruce Rheaume, University of Connecticut. CC-BY-4.0
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Immunostaining of retinal ganglion cells from the blind mole rat, Spalax Ehrenbergi, stained for RBPMS (red), melanopsin (green), and DAPI (blue). Credit: Jens Hannibal, Ass. Professor, MD, PhD., DmSc. Dept. Clinical Biochemistry, Bispebjerg Hospital, University of Copenhagen, Denmark.
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Immunofluorescence staining of flat-mounted mice retinas with RBPMS (green) at different times after NMDA injury. RGCs were quantified in three areas (center, middle, periphery) in each of the four quadrants of the retina depending on relative location to optic nerve head. Image from publication CC-BY-4.0. PMID: 36289519
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Immunostaining of Rbfox1 (red) , RBPMS (green), and DAPI (blue) in 22-month-old mouse retinas. Image from publication CC-BY-4.0. PMID: 36359797
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RBPMS staining (cat. 1832-RBPMS, 1:500; red) and eGFP staining (green in upper panels and grey in lower panels) in post-mortem retinal explants of C57BL/6J and B6.BOla-WldS mice that were transduced with AAV2-SYN-eGFP ex vivo. Mouse explants were stained for eGFP. Image from publication CC-BY-4.0. PMID: 36635457
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Confocal micrograph of melanopsin, TdTomato, and RBPMS (cat. 1832-RBPMS) co-staining in the dorsal GlyT2Cre;Ai9 mouse retina. The left arrows indicate RBPMS-positive GlyT2Cre ipRGCs. All melanopsin-negative, TdTomato-positive cells are RBPMS negative (arrowheads). Image from publication CC-BY-4.0. PMID: 36932080
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Immunostaining of the peripheral region of mouse retinal whole mount labeling RBPMS (Cat no 1832-RBPMS, 1:1500). Image kindly provided by Nicholas Tran, Harvard University.
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Immunostaining of mouse retinal ganglion cells (whole mount) labeling RBPMS (Cat no. 1832-RBPMS, 1:500, green). Image kindly provided by Sui Wang, Stanford University.
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Cryosections of wild type developing retinas at (A) E11.5 and (B) E12.5 were immunolabeled with anti-RBPMS (cat. 1832-RBPMS, 1:500; green) and anti-POU4F1 (red) and nuclear counterstained with DAPI (blue). Arrowheads indicate RBPMS expression and RBPMS-POU4F1 co-expression in the central retina ((B), left and central panels, respectively). Far right panels show the enlarged views of the boxed regions. Image from publication CC-BY-4.0. PMID: 37566030
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Representative images of retinal cryosections immunolabeled for RBPMS, tdTomato (RFP) and POU4F2 after induction by a single dosage of tamoxifen. IP injection of tamoxifen was administered at (C) E11.5, (D) E12.5, and (E) E13.5, and retinas were collected for immunolabeling 2 days after tamoxifen induction at E13.5, E14.5, and E15.5, respectively. DAPI (blue) was used to stain the nuclei. Far right panels show the enlarged views of the boxed regions. Image from publication CC-BY-4.0. PMID: 37566030
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Immunostaining of rat retinal ganglion cells showing specific immunolabeling of RBPMS(1:500) in green. Photo courtesy of Brian Choi, Univ. of Toronto.
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Immunostaining of mouse retina showing labeling of Rbfox1 (red), RBPMS (green), and DAPI(blue). Image from publication CC-BY-4.0. PMID: 35730583
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Immunostaining of GPX4 (red) in retinal ganglion cells (counterstained with RBPMS; green) in sham operation (SO) and ph-IOP injured mice, with or without DFP treatment. Image from publication CC-BY-4.0. PMID: 35933500
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Immunostaining of ACSL4 (red) in retinal ganglion cells (counterstained with RBPMS; green) in sham operation (SO) and ph-IOP injured mice, with or without DFP treatment. Image from publication CC-BY-4.0. PMID: 35933500
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Upper panel shows representative images of cross-sections of saline-injected mouse retinas and lower panel shows representative images of cross-sections of 50 ng Wnt5a-injected mouse retinas co-immunostained with phospho CamKII (green), RBPMS (red), and DAPI (blue). Image from publication CC-BY-4.0. PMID: 35914928
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Immunolabeling of E18.5 mouse retina specifically labeling RBPMS (1832-RBPMS, 1:500). Image produced by Marley Blommers and kindly provided by Angelo Iulianella, Dalhousie University. More information can be found in the publication "Retinal neuroblast migration and ganglion cell layer organization require the cytoskeletal-interacting protein Mllt11." Developmental Dynamics, PMID: 36131367.
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Immunostaining of mouse flat mounted retina showing specific immunolabeling of Anti-RBPMS (cat. 1832-RBPMS, 1:250). Photo courtesy of the Feldhem Lab at University of California, Santa Cruz.
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Western blot of rat heart lysate showing specific labeling of the ~24 kDa RBPMS protein.
| Manufacturer: | Phosphosolutions |
| Preservative: | 0.03% Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Guinea Pig |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to amino acid residues from the N-terminal region of rat RBPMS, conjugated to keyhole limpet hemocyanin (KLH). |
| Application: | ICC, WB, IHC |
Additional Text
Molecular Weight
24
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Uniprot ID
Q93062, Q9WVB0
Short Description
Our Anti-RBPMS primary antibody from PhosphoSolutions is rabbit polyclonal. It detects guinea pig, human, mouse, non-human primate, rabbit, and rat RBPMS and is antigen affinity purified. It is great for use in WB, IHC, ICC.
Specificity
FLJ32971 antibody, Heart and RRM expressed sequence antibody, Hermes antibody, RBP MS antibody, RBP-MS antibody, Rbpms antibody, RBPMS_HUMAN antibody, RNA binding protein gene with multiple splicing antibody, RNA-binding protein with multiple splicing antibody
Storage Note
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Gene ID
11030; 19663
Gene Name
Rbpms, RBPMS
1832-RBPMS Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations