SNAP-ChIP® OncoStat™ Panel
Catalogue Number: 19-2001-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | DNA Binding Proteins |
| Shipping Condition: | Blue Ice |
| Unit(s): | 20 ul |
Description
Description: A panel of distinctly modified mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3.3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.3-P84243*; H4-P62805) wrapped by 147 base pairs of barcoded Widom 601 positioning sequence DNA. *The mononucleosomes constitute a pool of 1 wild type (WT) unmodified plus 7 histone H3.3 point mutations: H3.3K4M, H3.3K9M, H3.3K27M, H3.3G34R, H3.3G34V, H3.3G34W, H3.3K36M. Each distinctly modified nucleosome is distinguishable by a unique sequence of DNA ("barcode") at the 3' end that can be deciphered by qPCR or next-generation sequencing. Each of the eight nucleosomes in the pool is wrapped by two DNA species, each containing a distinct barcode ("A" and "B", see SNAP-ChIP Manual) allowing for an internal technical replicate.
Additional Text
Application Notes
SNAP-ChIP OncoStat Panel consists of highly purified recombinant mononucleosomes and are suitable for use as spike-in controls for ChIP assays, for antibody specificity testing or for effector protein binding experiments. See manual for more information.
Storage Note
Stable for six months at -20°C from date of receipt.
19-2001 Datasheet
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