Strep-Tactin®XT Purification Buffer Set
Catalogue Number: 2-1043-000-IBA
| Manufacturer: | IBA GmbH |
| Type: | Protein Purification |
| Shipping Condition: | RT |
| Storage Condition: | 2-8°C |
| Unit(s): | 1 set |
Description
Description: Strep-Tactin®XT Buffer Set contains all buffers necessary for purification of Strep-tag®II or Twin-Strep-tag® fusion proteins with Strep-Tactin®XT as well as for subsequent regeneration of the resin. 10x Buffer W is a 10x concentrated wash buffer for Strep-Tactin® and Strep-Tactin®XT resins. It removes unbound molecules from the affinity chromatography matrix during Strep-tag®II and Twin-Strep-tag® fusion protein purification. 10x Buffer BXT is a 10x concentrated Strep-Tactin®XT elution buffer containing biotin. Biotin is a water-soluble B-complex vitamin, also known as vitamin B7 or vitamin H. It binds to Strep-Tactin®XT with very high affinity but reversibly and, thereby, allows the efficient elution of Strep-tag®II or Twin-Strep-tag® fusion proteins from the resin. Please note that 10x Buffer BXT is not applicable for elution of Strep-tag®II or Twin-Strep-tag® fusion proteins from Strep-Tactin®, since biotin irreversibly binds to these resins, and, thus, Strep-Tactin® cannot be regenerated. Buffer XT-R is a ready-to-use Strep-Tactin®XT regeneration buffer containing magnesium chloride for competitive displacement of biotin from the modified biotin-binding pocket of Strep-Tactin®XT after the elution of Strep-tag®II or Twin-Strep-tag® fusion proteins. Please note that the Strep-Tactin®XT Buffer Set is not applicable for Strep-Tactin®.
Additional Text
Short Description
Strep-Tactin®XT purification buffer set
2-1043-000 MSDS
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