pASK-IBA4C
Catalogue Number: 2-1323-000-IBA
| Manufacturer: | IBA GmbH |
| Shelf Life: | 12 Months |
| Type: | Vector |
| Shipping Condition: | RT |
| Storage Condition: | 2-8°C |
| Unit(s): | 5 ug |
Description
Description: pASK-IBA4C is a classic cloning vector devised for E. coli expression of recombinant proteins with the Strep-tag®II fused to the N-terminus. It carries the chloramphenicol resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, a multiple cloning site with recognition sites for several common restriction enzymes (e.g. EcoRI, SmaI, BamHI, SalI, PstI), the inducible tetracycline promoter/operator for the regulated expression of proteins, and the ompA signal for periplasmic secretion of the recombinant protein. The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. During the secretion into the periplasmic space the ompA signal sequence will be cleaved off.
Additional Text
Short Description
Periplasmic E. coli expression vector with N-terminal Strep-tag®II and chloramphenicol resistance
2-1323-000 Datasheet
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