Strep-Tactin® HRP conjugate

Catalogue Number: 2-1502-001-IBA

Manufacturer:IBA GmbH
Shelf Life:12 Months
Type:Avidin/Streptavidin Biotin Products
Shipping Condition:RT
Storage Condition:2-8°C
Unit(s): 0.5 ml

Description

Description: The streptavidin variant Strep-Tactin® is available as conjugate with horseradish peroxidase (HRP) or with alkaline phosphatase (AP) for fast and directed detection of N-terminally, C-terminally, or internally tagged Strep-tag®II or Twin-Strep-tag® fusion proteins transferred to a blotting membrane. Both conjugates are suitable for chromogenic reaction, whereas Strep-Tactin® HRP is applicable for chemiluminescence reaction as well. Depending on the host organism, protein extracts may contain small amounts of biotinylated proteins, e.g., the biotin carboxyl carrier protein (BCCP, 22 kDa ) of E. coli. Biotin Blocking Buffer is recommended for blocking of such biotinylated proteins on western blots prior to detection of Strep-tag®II or Twin-Strep-tag® fusion proteins with Strep-Tactin® HRP to prevent the detection of false positive bands. For this purpose, Biotin Blocking Buffer contains the tetrameric biotin binding protein avidin, which selectively binds biotinylated proteins.

Additional Text

Short Description

Strep-Tactin® conjugates to detect Strep-tag®II and Twin-Strep-tag®