Amplite® Fluorimetric cADP-Ribose Assay Kit
Catalogue Number: 20305-AAT
| Manufacturer: | AAT Bioquest |
| Type: | Detection Kit |
| Shipping Condition: | Dry Ice |
| Unit(s): | 100 tests |
Description
Description: cADP-ribose (cADPR) is a novel Ca2+ messenger derived from NAD+. ADP-riboxyl cyclase (ADPRC) catalyzes the synthesis of cADPR from NAD+, but the reaction can be reversed in the presence of high concentration of nicotinamide, producing NAD+ from cADPR stoichiometrically. The resultant NAD+ can be detected using our newly developed NAD sensor Quest Fluor™ NAD reagent. This makes monitoring cADPR in tissues and cell cultures possible in the low nM range. The NAD+ detection using Quest Fluor™ NAD reagent is specific to NAD+ and has no reaction to NADH. The fluorescent signal can be readily detected. This assay can be performed in a convenient 96-well or 384-well microtiter plate.
Additional Text
Storage Note
Multiple;|6 months
20305 Protocol
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