Fluo-4, Pentapotassium Salt
Catalogue Number: 20555-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Physical state: | Liquid |
| Type: | Calcium Indicator |
| Shipping Condition: | RT |
| Unit(s): | 1 mg |
Description
Description: Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. Cells may be physically loaded with the cell-impermeant salt forms of these indicators using patch pipette or microinjection. These indicators are useful for fluorescence and confocal microscopy, flow cytometry, and microplate screening applications.
Additional Text
Storage Note
Freeze (< -15 °C);|12 months
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