CUTANA™ Nuclei Extraction Buffer

Catalogue Number: 21-1026-EPC

Manufacturer:	EpiCypher
Shelf Life:	6 months
Physical state:	Protease inhibitor is not included. EpiCypher suggests using cOmplete™, EDTA-free Protease Inhibitor Cocktail (Roche 11873580001).
Type:	Biological Buffers
Shipping Condition:	Blue Ice
Storage Condition:	2-8°C
Unit(s):	100 reactions
Application:	CUT&RUN, CUT&Tag

Overview
Datasheets

Additional Text

Application Notes

Prepare Nuclei Extraction Buffer FRESH on day of use as outlined below. This master mix includes 20% extra volume to account for pipetting error; no additional buffer volume is needed. Per CUT&RUN or CUT&Tag reaction, combine: 235 µL Pre-Nuclei Extraction Buffer (21-1026a) 0.13 µL 1M Spermidine (21-1026b)* 9.8 µL 25X Protease Inhibitor (Roche, prepare by dissolving 1 tablet in 2 mL water)** *If preparing buffer for fewer than 8x reactions, dilute the 1M Spermidine stock 1:10 in molecular biology-grade water and add 1.3 µL per reaction. **Leftover 25X Protease Inhibitor can be stored for 12 weeks at -20C Follow instructions detailed in EpiCypher's CUTANA™ Nuclei Extraction Protocol for CUT&RUN and CUT&Tag (epicypher.com/protocols) to isolate nuclei from suspension and adherent cells. The protocol includes key quality control checks to ensure nuclei are high quality before starting CUT&RUN or CUT&Tag.

Purification

Protease inhibitor is not included. EpiCypher suggests using cOmplete™, EDTA-free Protease Inhibitor Cocktail (Roche 11873580001).

Storage Note

Store the Pre-Nuclei Extraction Buffer at 4C and the 1 M Spermidine at -20C. Components are stable for 6 months upon date of receipt.

21-1026 Datasheet

Price

£258.00

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