Catalogue Number: 21025-AAT
Manufacturer: | AAT Bioquest |
Shelf Life: | 12 months |
Type: | Fluorescent Reagents |
Shipping Condition: | RT |
Unit(s): | 1 mg |
Description: Among the ratiometric calcium indicators, Fura-2 and Indo-1 are most commonly used. Fura-2 is excitation-ratioable while Indo-1 is emission-ratioable. Fura-2 is preferred for ratio-imaging microscopy, in which it is more practical to change excitation wavelengths than emission wavelengths. Upon binding Ca2+, Fura-2 exhibits an absorption shift that can be observed by scanning the excitation spectrum between 300 and 400 nm, while monitoring the emission at ~510 nm. Fura-2 potassium salt is a cell-impermeable fluorescent probe for detection Ca2+ in a ratiometric mode.
Freeze (< -15 °C); Minimize light exposure;|12 months