Fura-FF, AM [Fura-2FF, AM] *CAS 348079-12-9*
Catalogue Number: 21027-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Fluorescent Reagents |
| Shipping Condition: | RT |
| Unit(s): | 10x50 ug |
Description
Description: Among the ratiometric calcium indicators, Fura-2 and Indo-1 are most commonly used. Fura-2 is excitation-ratioable while Indo-1 is emission-ratioable. Fura-2 is preferred for ratio-imaging microscopy, in which it is more practical to change excitation wavelengths than emission wavelengths. Upon binding Ca2+, Fura-2 exhibits an absorption shift that can be observed by scanning the excitation spectrum between 300 and 400 nm, while monitoring the emission at ~510 nm. The cell-permeant Fura-2FF AM is an analog of Fura-2 AM with much lower calcium binding affinity, Kd ~10 µM. This AM ester form can be loaded into live cells noninvasively.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|12 months
21027 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations