Fura-FF, pentapotassium salt [Fura-2FF, pentapotassium salt] *CAS 192140-58-2*
Catalogue Number: 21028-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Fluorescent Reagents |
| Shipping Condition: | RT |
| Unit(s): | 10x50 ug |
Description
Description: Among the ratiometric calcium indicators, Fura-2 and Indo-1 are most commonly used. Fura-2 is excitation-ratioable while Indo-1 is emission-ratioable. Fura-2 is preferred for ratio-imaging microscopy, in which it is more practical to change excitation wavelengths than emission wavelengths. Upon binding Ca2+, Fura-2 exhibits an absorption shift that can be observed by scanning the excitation spectrum between 300 and 400 nm, while monitoring the emission at ~510 nm. The cell-impermeant Fura-2FF is an analog of Fura-2 with much lower calcium binding affinity, Kd ~10 µM.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|12 months
21028 Datasheet
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