Fura-8™, pentasodium salt
Catalogue Number: 21058-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Fluorescent Reagents |
| Shipping Condition: | RT |
| Unit(s): | 1 mg |
Description
Description: Although Fura-2 has been widely used as the preferred excitation-ratioable calcium indicator it has certain limitations, e.g., lower sensitivity compared to the single wavelength calcium dyes such as Fluo-8® and Cal-520®. AAT has recently developed Fura-8™ to further improve the calcium response of Fura-2. Fura-8 has its emission shifted into longer visible wavelength that is compatible with the common filter sets. It has comparable calcium affinity (to Fura-2). Fura-8™ AM is more sensitive to calcium than Fura-2 AM with higher signal/background ratio than that of Fura-2 AM. We recommend to use Ex/Em = 354/530 nm and 415/530 nm for ratio measurements, i.e., calculating the excitation intensity ratios at 354 nm and 415 nm by monitoring emission intensity at 530 nm.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|12 months
21058 Datasheet
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