Anti-Vimentin Antibody
Catalogue Number: 2107-VIM-PPS
2107-VIM Image
2107-VIM Image
2107-VIM Image
2107-VIM Image
2107-VIM Image
2107-VIM Image
2107-VIM Image
Immunostaining of E20 rat cortical neuron/glial cell culture stained with anti-vimentin (cat. 2107-VIM, red, 1:2000) and anti-GFAP antibody (cat. 621-GFAP, green, 1:5000). The blue is DAPI staining nuclear DNA. Vimentin is expressed alone in fibroblastic and developing cells and appear red. The astrocytes that appear red express only GFAP, while the golden yellow and yellow astrocytes express GFAP and Vimentin.
2107-VIM Image
Mixed neuron/glial cultures stained with anti-vimentin (green, 1:500) and rabbit anti-GFAP antibody (cat. 620-GFAP, red, 1:1000). The blue stains nuclear DNA. Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin).
2107-VIM Image
Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa vimentin protein.
2107-VIM Image
Immunostaining of E20 rat cortical neuron/glial cell culture stained with anti-vimentin (cat. 2107,VIM, red, 1:2000) and anti-GFAP antibody ( cat.621-GFAP , green, 1:5000). The blue is DAPI staining nuclear DNA. Vimentin is expressed alone in fibroblastic and developing cells and appear red. The astrocytes that appear red express only GFAP, while the golden yellow and yellow astrocytes express GFAP and Vimentin.
2107-VIM Image
Mixed neuron/glial cultures stained with anti-vimentin (green, 1:500) and rabbit anti-GFAP antibody ( cat.620-GFAP , red, 1:1000). The blue stains nuclear DNA. Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin).
2107-VIM Image
Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa vimentin protein.
| Manufacturer: | Phosphosolutions |
| Preservative: | 10 mM Sodium azide |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | CTRCT30 antibody, Epididymis luminal protein 113 antibody, FLJ36605 antibody, HEL113 antibody, VIM antibody, VIME_HUMAN antibody, vimentin antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | 2D1 |
| Isotype: | IgG2a |
| Immunogen: | Recombinant human vimentin purified from E. coli. |
| Application: | ICC, WB, IHC |
Additional Text
Gene Name
VIM
Gene ID
7431
Molecular Weight
50
Uniprot ID
P08670
Purification
Protein G purified
Antibody Clonality
Monoclonal
Short Description
Our Anti-Vimentin primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Vimentin and is Protein G purified. It is great for use in WB, IHC, ICC.
Storage Note
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
2107-VIM Datasheet
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DELIVERY
we offer free delivery to UK universities and non profit organisations