CytoFix™ BCECF, AM *Optimized for long term cellular pH tracking*
Catalogue Number: 21200-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Cell Stains and Indicators |
| Shipping Condition: | RT |
| Unit(s): | 1 mg |
Description
Description: Intracellular pH plays an important modulating role in many cellular events, including cell growth, calcium regulation, enzymatic activity, receptor-mediated signal transduction, ion transport, endocytosis, chemotaxis, cell adhesion and other cellular processes. pH-sensitive fluorescent dyes have been widely applied to monitor changes in intracellular pH in recent years. Imaging techniques that use fluorescent pH indicators also allow researchers to investigate these processes with much greater spatial resolution and sampling density that can be achieved using other technologies such as microelectrode. Among them, 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) is the most popular pH probe since it can be used to monitor cellular pH ratiometrically with pKa ~7.0. BCECF AM is the cell-permeable version of BCECF. However, BCECF rapidly leaks out of lives cell when BCECF is loaded into live cells through its AM ester due to the activities of drug efflux transporters. CytoFix™ BCECF is a modified BCECF derivative that contains a cell-retention group. The newly modified BCECF derivative is well retained in live cells for much longer time than the parent BCECF dye. CytoFix™ BCECF retains the same pH profile of BCECF with improved cell retention.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|12 months
21200 Protocol
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