CytoCalcein™ Violet 450 *Excited at 405 nm*
Catalogue Number: 22012-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Cell Stains and Indicators |
| Shipping Condition: | RT |
| Unit(s): | 1 mg |
Description
Description: CytoCalcein™ Violet 450 is designed for labeling live cells in the same way to calcein AM. It has the maximum excitation at 405 nm that perfectly matches the violet laser line of flow cytometer, can also be well excited with the excitation sources of fluorescence microscopes. Upon getting into live cells the weakly fluorescent CytoCalcein™ Violet 450 is hydrolyzed into a strongly fluorescent dye that has the excitation/emission maxima 405 nm/450 nm. This exceptional spectral separation from the typical FACS fluorophores provides additional options for multiplexing experiments. Compared to calcein blue, CytoCalcein™ Violet 450 is brighter, and can be better excited with 405 nm laser line. CytoCalcein™ Violet 450, CytoCalcein™ Violet 500, CytoCalcein™ Blue 550 and CytoCalcein™ Blue 600 have been developed for flow cytometric applications. CytoCalcein™ dyes exhibit similar biological properties to Calcein, AM. They are optimized for the excitation wavelengths of a variety of flow cytometers, providing additional colors for flow cytometric analysis of live cells. CytoCalcein™ Violet 450 and CytoCalcein™ Violet 500 are well excited by 405 nm of violet laser and emit fluorescence at 450 nm and 500 nm respectively. CytoCalcein™ Blue 550 and CytoCalcein™ Blue 550 are well excited by 488 nm of blue laser and emit fluorescence at 550 nm and 600 nm respectively.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|12 months
22012 Protocol
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