Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit *Red Fluorescence*
Catalogue Number: 22797-AAT
| Manufacturer: | AAT Bioquest |
| Type: | Apoptosis Detection Kit |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 Tests |
Description
Description: Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring Caspase 3 activation. Caspase 3 is widely accepted as a reliable indicator for cell apoptosis since the activation of caspase-3 (CPP32/apopain) is important for the initiation of apoptosis. Caspase 3 has substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). This kit uses Z-DEVD-ProRed™ as the fluorogenic indicator for caspase-3 activity. Cleavage of ProRed™ DEVD blocking peptide residue by caspase 3 generates strongly red fluorescent ProRed™ that is monitored fluorimetrically at ~620 nm with excitation of ~530 nm. The kit provides all the essential components with an optimized assay protocol. The assay is robust, and can be readily adapted for high-throughput assays. Using 100 ul of reagents per well in a 96-well format, this kit provides sufficient reagents to perform 100 assays. Using 25 ul of reagents per well in a 384-well format, this kit provides sufficient reagents to perform 400 assays.
Additional Text
Storage Note
Multiple;|6 months
22797 Protocol
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations