Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Red Fluorescence*
Catalogue Number: 22817-AAT
| Manufacturer: | AAT Bioquest |
| Type: | Apoptosis Detection Kit |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 Tests |
Description
Description: Our Cell Meter™ assay kits are a set of tools for monitoring cellular functions. There are a variety of parameters that can be used. This particular kit is designed to monitor cell apoptosis by measuring caspase 9 activity. Caspase 9 is a member of the CED-3 subfamily. Activated Caspase-9 cleaves downstream caspases such as caspase-3, -6 and -7, initiating the caspase cascade. It is essential for apoptosis during normal development of the central nervous system. Caspase 9 is proven to have selectivity for the peptide sequence Leu-Glu-His-Asp (LEHD). This kit uses Ac-LEHD-ProRed™ as a fluorogenic indicator for caspase 9 activity. Cleavage of ProRed™ by caspase 9 generates strongly fluorescent ProRed™ which is monitored at Ex/Em = 530/620 nm. The kit provides all the essential components. The assay is robust and can be readily adapted for high throughput screening. It can be used to either quantify the activated caspase 9 activities in apoptotic cells or screen the caspase 9 inhibitors. Quite a few labs have used this kit for high throughput screenings.
Additional Text
Storage Note
Multiple;|6 months
22817 Protocol
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