Phalloidin-iFluor® 350 Conjugate
Catalogue Number: 23110-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Probes |
| Shipping Condition: | Blue Ice |
| Unit(s): | 300 Tests |
Description
Description: This blue fluorescent phalloidin conjugate (equivalent to Alexa Fluor® 350-labeled phalloidin) selectively binds to F-actins. Phalloidin derivatives are convenient probes used at nanomolar concentrations for labeling, identifying, and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures, or cell-free experiments. Phalloidin binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the rate constant for the dissociation of actin subunits from filament ends, essentially stabilizing actin filaments through the prevention of filament depolymerization. Moreover, phalloidin is found to inhibit the ATP hydrolysis activity of F-actin. Phalloidin functions differently at various concentrations in cells. When introduced into the cytoplasm at low concentrations, phalloidin recruits the less polymerized forms of cytoplasmic actin and filamin into stable islands" of aggregated actin polymers. Yet
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|12 months
23110 Protocol
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