Anti-GAPDHS Monoclonal Antibody (Clone:Hs-8)
Catalogue Number: 30-1189-ABO
| Manufacturer: | Abeomics |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | GAPDHS||GAPD2||GAPDH2||GAPDS||HSD-35||HSD35 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 0.1 mg |
| Host name: | Mouse |
| Clone: | Hs-8 |
| Isotype: | IgM |
| Immunogen: | Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. |
| Application: | FACS, ICC, WB |
Description
Description: GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
Additional Text
Gene Name
GAPDHS
Storage Note
Store at 2-8°C. Do not freeze.
Gene ID
26330
Uniprot ID
O14556
Purification
Purified
Antibody Clonality
Monoclonal
Application Notes
Western Blotting Immunocytochemistry Recommended dilution: Purified Antibody: 10 µg/ml Staining technique: Membrane permeabilization (acetone) is essential. Flow Cytometry
30-1189 Datasheet
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