Anti-beta-tubulin / TUBB Monoclonal Antibody (Clone:TU-13)

Catalogue Number: 30-1306-ABO

Manufacturer:Abeomics
Type:Monoclonal Primary Antibody - Unconjugated
Alias:Tubulin beta-5 chain, Tubb5
Shipping Condition:Blue Ice
Unit(s): 0.1 mg
Host name: Mouse
Clone: TU-13
Isotype: IgM
Immunogen: beta-tubulin from porcine brain
Application: WB

Description

Description: The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening - this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains.The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.

Additional Text

Gene Name

TUBB

Gene ID

203068

Storage Note

Store at 2-8°C. Do not freeze.

Uniprot ID

P07437

Purification

Purified

Antibody Clonality

Monoclonal

Application Notes

Western blotting: Recommended dilution: 1-2 µg/ml, reducing conditions.