Mouse IgG3 Isotype Control Monoclonal Antibody (Clone:PPV-07)
Catalogue Number: 30-1319-ABO
| Manufacturer: | Abeomics |
| Type: | Isotype control - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 0.1 mg |
| Host name: | Mouse |
| Clone: | PPV-07 |
| Isotype: | IgG3 |
| Immunogen: | |
| Application: | FACS |
Description
Description: The specificity of staining by Monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 µg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific Monoclonal antibody used in a particular immunoassay. The Monoclonal antibody PPV-07, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG3 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Additional Text
Purification
Protein A purified
Antibody Clonality
Monoclonal
Application Notes
Flow Cytometry Control Experiments The antibody is suitable for control experiments when performing cell surface staining as well as intracellular staining.
Storage Note
Store at 2-8°C. Do not freeze. HEPES buffered saline (HBS), pH 7.0, 15 mM sodium azide
30-1319 Datasheet
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