Anti-gamma-tubulin 1 Monoclonal Antibody (Clone:TU-30)
Catalogue Number: 30-1323-ABO
30-1323 Image
30-1323 Image
30-1323 Image
30-1323 Image
30-1323 Image
Figure 1: Immunofluorescence staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3). Nuclei were stained with DAPI (blue).
30-1323 Image
Figure 2: Immunofluorescence staining of mouse fibroblasts using anti-gamma-tubulin (TU-30; direct conjugate with Dyomics 547, red). Nuclei were stained with DAPI (blue). Fig. 3A, 3B, 3C Immunofluorescence staining (mouse fibroblasts) Fig. 3. Immunofluorescence staining of microtubular networks in 3T3 mouse fibroblasts. 3A - metaphase; 3B - anaphase; 3C - telophase Gamma-tubulin (red) stained with anti-gamma-tubulin (TU-30), alpha-tubulin (green) with polyclonal anti-alpha-tubulin antibody and nuclei with DAPI (blue).
30-1323 Image
Figure 3: Immunofluorescence staining of microtubular networks in 3T3 mouse fibroblasts. 3A - metaphase; 3B - anaphase; 3C - telophase Gamma-tubulin (red) stained with anti-gamma-tubulin (TU-30), alpha-tubulin (green) with polyclonal anti-alpha-tubulin antibody and nuclei with DAPI (blue).
30-1323 Image
Figure 4: Differential reactivity of monoclonal antibodies to gamma-tubulin-tubulin with human gamma-tubulin-tubulin isotypes.
| Manufacturer: | Abeomics |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | TUBG1||TUBG |
| Shipping Condition: | Blue Ice |
| Unit(s): | 0.1 mg |
| Host name: | Mouse |
| Clone: | TU-30 |
| Isotype: | IgG1 |
| Immunogen: | C-terminal peptide of gamma-tubulin counjugated to KLH. |
| Application: | ICC |
Description
Description: The gamma-tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface. Humans possess two gamma-tubulin genes. Gamma-tubulin 1 represents a ubiquitous isotype, whereas gamma-tubulin 2 is found predominantly in the brain, where it may be endowed with divergent functions beyond microtubule nucleation.
Additional Text
Gene ID
7283
Gene Name
TUBG1
Storage Note
Store at 2-8°C. Do not freeze.
Uniprot ID
P23258
Purification
Protein A purified
Antibody Clonality
Monoclonal
Application Notes
Immunocytochemistry: Recommended dilution: 1-2 µg/ml. Staining technique: (a) Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) Fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: P-19 murine embryonal carcinoma cell line, 3T3 murine fibroblasts. The antibody TU-30 stains only fixed cells. Western blotting: Recommended dilution 1-2 µg/ml, reducing conditions.
30-1323 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations