Anti-GST / Glutathione-S-Transferase, GST tag Monoclonal Antibody (Clone:S-tag-05)
Catalogue Number: 30-1331-ABO
30-1331 Image
30-1331 Image
Figure 1: Isolation of Glutathione-S-Transferase (GST) overexpressed in E. coli.Western blot was immunostained by anti-GST (S-tag-05). Lane 1: affinity purification of GST from bacterial cell lysate using commercial sorbent Glutathione-Sepharose. Lane 2: affinity purification of GST from bacterial cell lysate using immunosorbent prepared from anti-GST (S-tag-02; )
Description: The GST (Glutathione-S-Transferase) is a 26kDa protein encoded by the parasite Schistosoma japonicum, easily purified by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors. Antibodies against GST will detect the expressed GST protein or a GST-fusion protein, and can be utilized for immuno-purification of GST-fusion proteins.
Additional Text
Storage Note
Store at 2-8°C. Do not freeze.
Purification
Protein A purified
Antibody Clonality
Monoclonal
Application Notes
Western Blotting Recommended dilution: 1 µg/ml Positive control: GST fusion protein