Figure 1: Western Blotting analysis (reducing conditions) of recombinant protein Orf1 in cell lysate of Orf1-transfected E. coli using polyclonal anti-Orf1 antibody.
Slices containing purified FrpD250-6xHis-tagged protein were excised from 10% SDS-PAGE gels, grinded, mixed with 1 mg/ml of aluminum hydroxide in PBS, and used to immunize a rabbit.
Description: Orf1 (FrpD) is a Neisseria meningitidis outer membrane lipoprotein. Subcellular localization and [3H]palmitic acid labeling in Escherichia coli revealed that FrpD is synthesized with a type II signal peptide for export across the cytoplasmic membrane and is, upon processing to a lipoprotein, sorted to the outer bacterial membrane.
Additional Text
Storage Note
Store at 2-8°C. Do not freeze.
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Application Notes
Western Blotting Recommended dilution: 0.2 - 1 µg/ml