Anti-Actin Monoclonal Antibody (Clone:HHF35)
Catalogue Number: 30-1421-ABO
30-1421 Image
30-1421 Image
30-1421 Image
Figure 1: Western blotting analysis of muscle-specific actin using the monoclonal antibody HHF35. Lane 1: murine femoral muscle. Lane 2: murine heart. Lane 3: HeLa. Lane 4: HUVEC
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Figure 2: Immunohistochemistry staining of human muscle (paraffin-embedded sections) with anti-muscle-specific actin (HHF35).
| Manufacturer: | Abeomics |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Alpha-actin-1, ACTA |
| Shipping Condition: | Blue Ice |
| Unit(s): | 0.1 mg |
| Host name: | Mouse |
| Clone: | HHF35 |
| Isotype: | IgG1 |
| Immunogen: | SDS extracted protein fraction of human myocardium |
| Application: | WB |
Description
Description: Actin is a highly conserved ubiquitous globular protein (G-actin) that polymerizes to form fibrous F-actin microfilaments. In higher eucaryotes several actin isoforms have been identified, that fall into three classes. Alpha actin is a structural component of the contractile apparatus of muscle cells or muscle-derived cells. Beta actin and gamma actin play roles in regulation of cell motility in other cell types. Specific subcellular structures such as as stress fibers, focal adhesions, filopodia etc., are formed by involvement of actin cytoskeleton.
Additional Text
Gene ID
58
Gene Name
ACTA1
Uniprot ID
P68133
Storage Note
Store at 2-8°C. Do not freeze.
Purification
Protein A purified
Antibody Clonality
Monoclonal
Application Notes
Western Blotting Recommended dilution:1 µg/ml Positive control:murine femoral muscle, murine heart Negative control:HUVEC line Immunohistochemistry
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