Anti-Biotin Mab(1D4-C5)
Catalogue Number: 30-2889-ABO
30-2889 Image
30-2889 Image
Figure 1: ELISA analysis of a biotinylated antigen using biotin-specific antibody and streptavidin. Microtitration wells were coated with biotinylated human IgG (0.01 µg/ml) and blocked. Then either anti-biotin mouse monoclonal 1D4-C5 (A, C) or streptavidin-HRP (B) was added in six different doses. Finally, goat anti-mouse-HRP conjugate (A) or streptavidin-HRP (C) was added. HRP signal increased in a dose-dependent manner, but with different dynamics when the biotinylated antibody was detected by anti-biotin mAb/goat anti-mouse-HRP (A) or by streptavidin-HRP (B), and it was falling to zero in a dose-dependent manner when streptavidin-HRP was competed by anti-biotin mAb 1D4-C5 (C).
| Manufacturer: | Abeomics |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | vitamin H, vitamin B7 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 0.1 mg |
| Host name: | Mouse |
| Clone: | 1D4-C5 |
| Isotype: | IgG2a, kappa |
| Immunogen: | biotinylated limulus hemocyanin |
| Application: | ELISA, FACS, IP, MC |
Description
Description: Specificity: Mouse monoclonal antibody 1D4-C5 recognizes biotin and can be used as a second-step reagent for detecting biotinylated antigens.
Additional Text
Storage Note
Store at 2-8°C. Do not freeze.
Purification
Protein A purified
Antibody Clonality
Monoclonal
Application Notes
FC (QC tested), ELISA, IP, MC (CyTOF)
30-2889 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations