Peroxidase-conjugated AffiniPure™ Rabbit Anti-His Tag

Catalogue Number: 300-035-240-JIR

Manufacturer:	Jackson ImmunoResearch
Shelf Life:	12 months
Preservative:	No preservative
Physical state:	Freeze-dried solid
Type:	Tag antibodies
Shipping Condition:	RT
Unit(s):	0.1 ml
Host name:	Rabbit
Clone:
Isotype:
Immunogen:	His Tag
Application:	ELISA, ICC, WB, IHC

Overview
Datasheets

Description

Description: Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Based on antigen-binding assay and/or ELISA, the antibody reacts with proteins containing N-terminal or C-terminal His Tags (His6-His10).

Additional Text

Antibody Clonality

Polyclonal

Short Description

Rabbit Anti-His Tag secondary antibody, conjugated to Horseradish Peroxidase. This is an affinity purified whole IgG polyclonal antibody.

Application Notes

1:500 - 1:5,000 for immunohisto/cytochemistry 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:5,000 - 1:50,000 for Western blotting with ECL substrates Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.