Alexa Fluor® 647-conjugated AffiniPure™ Rabbit Anti-His Tag
Catalogue Number: 300-605-240-JIR
| Manufacturer: | Jackson ImmunoResearch |
| Shelf Life: | 12 months |
| Preservative: | 0.05% Sodium azide |
| Physical state: | Freeze-dried solid |
| Type: | Tag antibodies |
| Shipping Condition: | RT |
| Unit(s): | 0.1 mg |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | |
| Immunogen: | His Tag |
Description
Description: Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Based on antigen-binding assay and/or ELISA, the antibody reacts with proteins containing N-terminal or C-terminal His Tags (His6-His10).
Additional Text
Antibody Clonality
Polyclonal
Short Description
Rabbit Anti-His Tag secondary antibody, conjugated to Alexa Fluor® 647. This is an affinity purified whole IgG polyclonal antibody.
Application Notes
1:100 - 1:800 for most applications Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Storage Note
Store freeze-dried solid at 2-8°C.|One year from date of rehydration.
Guide to Immunoglobulins Structure and Function
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