Peroxidase-conjugated AffiniPure™ Rabbit Anti-Mouse IgG, Fcγ Fragment Specific (min X Hu Sr Prot)

Catalogue Number: 315-035-046-JIR

Manufacturer:	Jackson ImmunoResearch
Shelf Life:	12 months
Preservative:	No preservative
Physical state:	Freeze-dried solid
Type:	Polyclonal Secondary Antibody - Conjugated
Shipping Condition:	RT
Unit(s):	1 ml
Host name:	Rabbit
Clone:
Isotype:
Immunogen:	IgG, Fc fragment
Application:	ELISA, ICC, WB, IHC

Overview
Datasheets

Description

Description: Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of mouse IgG heavy chain but not with the Fab portion of mouse immunoglobulins. No antibody was detected against mouse IgM or non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human serum proteins, but it may cross-react with immunoglobulins from other species.

Additional Text

Purification

AffiniPure

Antibody Clonality

Polyclonal

Short Description

Rabbit Anti-Mouse IgG, Fc fragment secondary antibody, conjugated to Horseradish Peroxidase. This is an affinity purified whole IgG polyclonal antibody and has been tested and/or adsorbed to ensure minimal cross reactivity with Human serum proteins.

Application Notes

1:500 - 1:5,000 for immunohisto/cytochemistry 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000 - 1:200,000 for Western blotting with ECL substrates Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.