Polyclonal Antibody to Cherry Dsred
Catalogue Number: 34-1023-ABO
34-1023 Image
34-1023 Image
34-1023 Image
Figure-1: HEK293 cells transfected with a mCherry, stained with (34-1023) antibody and viewed in a confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red, and staining with (34-1023) is shown in Green. The green antibody staining is only seen cells which express mCherry, as expected, and the superimposition of the green and red results in an orange signal. Interestingly stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules releasing the low molecular weight mCherry fluorochrome.
34-1023 Image
Figure-2: Western blot analysis of HEK293 cell lysates, and recombinant protein solutions using chicken pAb to mCherry,(34-1023), dilution 1:2,000, in green [1] protein standard, [2] HEK293, [3] HEK293 cells transfected with an mCherry-HA constract, [4] pure recombinant mCherry protein, [5] pure recombinant GFP protein and [6] HEK293 transfected with a GFP construct. The major band at about 30kDa corresponds to mCherry-HA protein and the slightly larger recombinant form runs at about 33kDa due to presence of a His tag and other vector derived sequence. (34-1023) antibody recognizes mCherry protein and does not react with GFP protein. The same blot was simultaneously probed with mouse mAb to HSP60, dilution 1:10,000 in red, which reveals a band at 60kDa only in the cell lysates.
| Manufacturer: | Abeomics |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ul, 50 ul |
| Host name: | Chicken |
| Clone: | |
| Isotype: | IgY |
| Immunogen: | Full length recombinant protein |
| Application: | ICC, IF, WB, IHC |
Additional Text
Antibody Clonality
Polyclonal
UniProt ID
X5DSL3
Application Notes
WB: 1:2,000-5,000 IF/IHC: 1:1,000.
Storage Note
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
34-1023 Datasheet
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