Polyclonal Antibody to Cherry Dsred
Catalogue Number: 34-1024-ABO
34-1024 Image
34-1024 Image
34-1024 Image
Figure-1: Immunofluorescent analysis of HEK293 cells transfected with mCherry-HA, construct, in red, and stained with rabbit pAb to mCherry,(34-1024), dilution 1:1,000, in green. The blue is Hoechst staining of nuclear DNA. (34-1024) antibody reveals mCherry protein expressed only in transfected cells which appear golden in color. Untransfected cells do not react with the antibody, as a result only their nuclei are visible.
34-1024 Image
Figure-2: Western blot analysis of HEK293 cell lysates, and recombinant protein solutions using rabbit pAb to mCherry,(34-1024), dilution 1:1,000, in green [1] protein standard, [2] HEK293, [3] HEK293 cells transfected with mCherry-HA construct, [4] mCherry recombinant protein, [5] GFP recombinant protein, and [6] HEK293 transfected with a full length GFP construct. A major band at about 28kDa corresponds to mCherry protein. The (34-1024) antibody does not react with GFP protein. The same blot was simultaneously probed with chicken pAb to HSP60, dilution 1:5,000 in red which reveals a band at 60kDa only in cell lysates.
| Manufacturer: | Abeomics |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ul, 50 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Full length recombinant protein |
| Application: | ICC, IF, WB, IHC |
Additional Text
Antibody Clonality
Polyclonal
UniProt ID
X5DSL3
Application Notes
WB: 1:1,000 IF/IHC: 1:500.
Storage Note
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
34-1024 Datasheet
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