Monoclonal Antibody to Cherry Dsred (Clone: 1C51)
Catalogue Number: 34-1025-ABO
34-1025 Image
34-1025 Image
34-1025 Image
Figure-1: Immunofluorescent analysis of HEK293 cells transfected with mCherry-HA, construct, in red, and stained with mouse mAb to mCherry, (34-1025), dilution 1:500, in green. The blue is Hoechst staining of nuclear DNA. (34-1025) antibody reveals mCherry protein expressed only in transfected cells which appear golden in color. Untransfected cells do not react with the antibody, as a result only their nuclei are visible.
34-1025 Image
Figure-2: Western blot analysis of HEK293 cell lysates, and recombinant protein solutions using mouse mAb to mCherry, (34-1025), dilution 1:1,000, in green [1] protein standard, [2] HEK293, [3] HEK293 cells transfected with mCherry-HA construct, [4] mCherry recombinant protein, [5] GFP recombinant protein, and [6] HEK293 transfected with GFP construct. Major band at about 30kDa corresponds to mCherry protein. (34-1025) antibody does not react with GFP protein. The same blot was simultaneously probed with chicken pAb to HSP60, dilution 1:5,000 in red which reveals band at 60kDa seen only in cell lysates.
| Manufacturer: | Abeomics |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ul, 50 ul |
| Host name: | Mouse |
| Clone: | 1C51 |
| Isotype: | IgG2a |
| Immunogen: | Full length recombinant protein |
| Application: | ICC, IF, WB, IHC |
Additional Text
Antibody Clonality
Monoclonal
UniProt ID
X5DSL3
Application Notes
WB: 1:2,000 IF/IHC: 1:500.
Storage Note
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
34-1025 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations