Monoclonal Antibody to DCX Doublecortin (Clone: 3E1)
Catalogue Number: 34-1028-ABO
34-1028 Image
34-1028 Image
34-1028 Image
Figure-1: Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to doublecortin, (34-1028), dilution 1:1,000 in red, and costained with chicken pAb to microtubule associated protein 2 (MAP2),(34-1064), dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA. The doublecortin antibody reveals strong cytoplasmic staining in a population of small developing neurons and their processes, while the MAP2 antibody stains dendrites and perikarya of mature neurons. Doublecortin antibody is an excellent marker of early developing neuronal cells.
34-1028 Image
Figure-2: Western blot analysis of rat whole brain lysates using mouse mAb to doublecortin, (34-1028), dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain. Strong bands at 40kDa and 45kDa correspond to the doublecortin protein, detected only in the developing brain.
| Manufacturer: | Abeomics |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Doublin||Lis-X||Lissencephalin-X |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ul, 50 ul |
| Host name: | Mouse |
| Clone: | 3E1 |
| Isotype: | IgG2a |
| Immunogen: | Recombinant full length human Lis-A isoform of DCX purified from E. coli. |
| Application: | ICC, IF, WB, IHC |
Additional Text
Gene ID
1641
Gene Name
DCX
Uniprot ID
O43602
Antibody Clonality
Monoclonal
Application Notes
WB: 1:1,000. IF/ICC and IHC: 1:1,000.
Storage Note
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
34-1028 Datasheet
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