Monoclonal Antibody to Fibrillarin/Nop1p (Clone: 38F3)
Catalogue Number: 34-1031-ABO
34-1031 Image
34-1031 Image
34-1031 Image
Figure-1: High magnification confocal image of HeLa cells stained with fibrillarin antibody (34-1031), dilution 1:100 in green, and costained with(34-1126), chicken polyclonal antibody to vimentin, in red, 1:10,000. Nuclear DNA is revealed with the DAPI stain in blue. The fibrillarin antibody shows strong staining of nucleoli in the nucleus, while the vimentin antibody reveals cytoplasmic intermediate filaments.
34-1031 Image
Figure-2: Western blot analysis of lysates of cell fractions probed with mouse mAb to fibrillarin,(34-1031), dilution 1:500 in green: [1] protein standard, [2] C6 cytosol, [3] C6 nuclear, [4] HEK293 cytosol, [5] HEK293 nuclear, [6] NIH-3T3 cytosol and [7] NIH-3T3 nuclear fractions. The band at 37kDa corresponds to the fibrillarin protein detected exclusively in the nuclear fractions.