Polyclonal Antibody to GAP43
Catalogue Number: 34-1038-ABO
34-1038 Image
34-1038 Image
34-1038 Image
Figure-2: Western blot analysis of different tissue and cell lysates using chicken pAb to GAP43,(34-1038), dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. Single band at the 43kDa mark corresponds to GAP43. The GAP43 protein only is detected in the lysates of neuronal origin. C6 cells are a rat glioma cell line and do not express GAP43.
34-1038 Image
Figure-1: Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to GAP43,(34-1038), dilution 1:2,000 in green, and costained with rabbit pAb to a-II spectrin, (34-1109), dilution 1:1,000 in red. The blue is DAPI staining of nuclear DNA. GAP43 antibody labels protein expressed in the axonal membrane and synapses of neuronal cells, while the spectrin antibody stains the submembraneous cytoskeleton of axons and dendrites.
| Manufacturer: | Abeomics |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Axonal membrane protein GAP-43||Growth-associated protein 43||Neural phosphoprotein B-50||pp46 |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ul, 50 ul |
| Host name: | Chicken |
| Clone: | |
| Isotype: | IgY |
| Immunogen: | C-terminal peptide of rat and mouse GAP43, which is KEDPEADQEHA, to which an N terminal Cys residue was added to allow chemical coupling to KLH |
| Application: | ICC, IF, WB, IHC |
Additional Text
Gene Name
GAP43
Gene ID
2596
Uniprot ID
P17677
Antibody Clonality
Polyclonal
Application Notes
WB: 1:1,000-1-:5,000. IF/IHC: 1:500-1:1,000
Storage Note
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
34-1038 Datasheet
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