Monoclonal Antibody to GAP43 (Clone: 5E8)
Catalogue Number: 34-1040-ABO
34-1040 Image
34-1040 Image
34-1040 Image
Figure-1: Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to GAP43,(34-1040), dilution 1:1,000, in red, and costained with chicken pAb to GFAP,(34-1046), dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA. GAP43 antibody labels protein expressed in the axonal membrane of neuronal cells, while the GFAP antibody stains astroglial cells.
34-1040 Image
Figure-2: Western blot analysis of tissue and cell lysates using mouse mAb to GAP43,(34-1040), dilution 1:2,000, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. The single band at 43kDa corresponds to GAP43 protein. The protein is expressed in rodent and human neurons and neuronal derived cells but not in C6 cells which are of glial origin. Note that the protein from SH-SY5Y cells, the only line of human origin tested here, has a slightly lower SDS-PAGE than the rodent proteins.
| Manufacturer: | Abeomics |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Axonal membrane protein GAP-43||Growth-associated protein 43||Neural phosphoprotein B-50||pp46 |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ul, 50 ul |
| Host name: | Mouse |
| Clone: | 5E8 |
| Isotype: | IgG1 |
| Immunogen: | Recombinant full- length Human GAP43 |
| Application: | ICC, IF, WB, IHC |
Additional Text
Gene Name
GAP43
Gene ID
2596
Uniprot ID
P17677
Antibody Clonality
Monoclonal
Application Notes
WB: 1:1,000-5,000 IF/ICC and IHC: 1:1,000-5,000
Storage Note
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
34-1040 Datasheet
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