Polyclonal Antibody to Glial Fibrillary Acidic Protein,GFAP
Catalogue Number: 34-1042-ABO
34-1042 Image
34-1042 Image
34-1042 Image
Figure-1: Immunofluorescent analysis of a rat cerebellum section stained with rabbit pAb to GFAP,(34-1042), dilution 1:5,000 in green and costained with mouse mAb to MeCP2, dilution 1:500, in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 1 hour, cut to 45µM, and free-floating sections were stained with above antibodies. The GFAP antibody stains the network of astrocytic cells and the processes of Bergmann glia in the molecular layer. The MeCP2 antibody specifically labels nuclei of certain neurons.
34-1042 Image
Figure-2: Western blot analysis of different tissue lysates using rabbit polyclonal antibody to GFAP,(34-1042), dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Strong band at about 50kDa corresponds to the major isotype of the GFAP protein. Smaller isotypes and proteolytic fragments of GFAP are also detected on the blot.
| Manufacturer: | Abeomics |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ul, 50 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant human full length GFAP protein |
| Application: | ICC, IF, WB, IHC |
Additional Text
Gene Name
GFAP
Gene ID
2670
Uniprot ID
P14136
Antibody Clonality
Polyclonal
Application Notes
WB: 1:5,000 IF/ICC and IHC 1:1,000-1,5,000
Storage Note
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
34-1042 Datasheet
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