Polyclonal Antibody to Glial Fibrillary Acidic Protein, GFAP
Catalogue Number: 34-1046-ABO
34-1046 Image
34-1046 Image
34-1046 Image
Figure-1: Immunofluorescent analysis of a section of mouse hippocampus stained with chicken pAb to GFAP,(34-1046), dilution 1:5,000 in green and costained with rabbit pAb to FOX3/NeuN,(34-1036), dilution 1:5,000, in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, mouse brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. The GFAP antibody stains a network of astroglial cells while the Fox3/NeuN antibody stains the nuclei and proximal perikarya of neurons.
34-1046 Image
Figure-2: Western blot analysis of whole brain lysates using chicken pAb to GFAP,(34-1046), dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain. The strong band at about 50 kDa corresponds to the GFAP protein. Smaller proteolytic fragments and alternate transcripts of GFAP may also be detected on such blots.
| Manufacturer: | Abeomics |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ul, 50 ul |
| Host name: | Chicken |
| Clone: | |
| Isotype: | IgY |
| Immunogen: | Native GFAP, purified from bovine spinal cord |
| Application: | ICC, IF, WB, IHC |
Additional Text
Gene Name
GFAP
Gene ID
2670
Uniprot ID
P14136
Antibody Clonality
Polyclonal
Application Notes
WB: 1:5,000. IF/ICC and IHC: 1:1,000-1:5,000
Storage Note
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
34-1046 Datasheet
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