Monoclonal Antibody to Glyceraldehyde 3-Phosphate Dehydrogenase (Clone: 1D4)
Catalogue Number: 34-1047-ABO
34-1047 Image
34-1047 Image
34-1047 Image
Figure-1: Immunofluorescent analysis =of HeLa cells stained with mouse mAb to GAPDH,(34-1047), dilution 1:100 in green. Blue is Hoechst staining of nuclear DNA. The (34-1047) antibody produces strong cytoplasmic staining of healthy cells.
34-1047 Image
Figure-2: Western blot analysis of cell line lysates probed with mouse mAb to GAPDH,(34-1047), dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a "house keepinga protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.
| Manufacturer: | Abeomics |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Peptidyl-cysteine S-nitrosylase GAPDH |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 500 ul, 50 ul |
| Host name: | Mouse |
| Clone: | 1D4 |
| Isotype: | IgM |
| Immunogen: | Whole protein purified from pig |
| Application: | ICC, IF, WB, IHC |
Additional Text
Gene Name
GAPDH
Uniprot ID
P04406
Gene ID
2597
Antibody Clonality
Monoclonal
Application Notes
WB: 1:1,000 IF/IHC: 1:100.
Storage Note
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
34-1047 Datasheet
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