Polyclonal Antibody to Glyceraldehyde 3-Phosphate Dehydrogenase
Catalogue Number: 34-1048-ABO
34-1048 Image
34-1048 Image
34-1048 Image
Figure-1: Immunofluorescent analysis of HeLa cells stained with rabbit pAb to GAPDH,(34-1048), dilution 1:2,000 in red. Blue is Hoescht staining of nuclear DNA. The (34-1048) antibody produces diffuse cytoplasmic staining of cells.
34-1048 Image
Figure-2: Western blot analysis of different cell cytosolic or nuclear enriched fractions, using rabbit pAb to GAPDH,(34-1048), dilution 1:20,000, in red: [1] protein standard, [2] NIH-3T3 cytosolic, [3] NIH-3T3 nuclear, [4] HeLa cytosolic, and [5] HeLa nuclear fractions. Strong band at 37kDa corresponds to GAPDH protein, mainly detected in the cytosolic fractions. The same blot was simultaneously probed with mouse mAb to the nuclear RNA binding protein SF3B4,(34-1113), dilution 1:1,000, in green. In contrast to GAPDH, SF3B4 is exclusively expressed in the nuclear fraction.
| Manufacturer: | Abeomics |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Peptidyl-cysteine S-nitrosylase GAPDH |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ul, 50 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Native porcine purified protein |
| Application: | ICC, IF, WB, IHC |
Additional Text
Gene Name
GAPDH
Uniprot ID
P04406
Gene ID
2597
Antibody Clonality
Polyclonal
Application Notes
WB: 1:30,000 IF/ICC and IHC: 1:2,000
Storage Note
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
34-1048 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations