Monoclonal Antibody to a-internexin/NF66 (Clone: 1D2)
Catalogue Number: 34-1052-ABO
34-1052 Image
34-1052 Image
34-1052 Image
Figure-1: Immunofluorescent analysis of rat cerebellum section stained with mouse mAb to a-internexin,(34-1052), dilution 1:5,000 in green, and costained with chicken pAb to calretinin, 1:2,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. The a-internexin antibody selectively stains neuronal processes, in particular parallel fibers, the axons of granule cells. Calretinin antibody stains interneurons predominantly in the molecular layer of the cerebellum.
34-1052 Image
Figure-2: Western blot analysis of different tissue lysates using mouse mAb to a-internexin,(34-1052), dilution 1:10,000 in green: [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig spinal cord and [7] cow spinal cord.(34-1052) antibody reveals the a-internexin protein with apparent molecular weight of 64 to 66 kDa with slight variability among species.
| Manufacturer: | Abeomics |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | 66 kDa neurofilament protein||Neurofilament 5 |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 500 ul, 50 ul |
| Host name: | Mouse |
| Clone: | 1D2 |
| Isotype: | IgG1 |
| Immunogen: | Purified recombinant rat Alpha -internexin expressed in and purified from E. coli. |
| Application: | ICC, IF, WB, IHC |
Additional Text
Gene ID
9118
Gene Name
INA
Uniprot ID
Q16352
Antibody Clonality
Monoclonal
Application Notes
WB: 1:10,000. IF/ICC and IHC: 1:5,000.
Storage Note
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
34-1052 Datasheet
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