Polyclonal Antibody to a-internexin/NF66
Catalogue Number: 34-1054-ABO
34-1054 Image
34-1054 Image
34-1054 Image
Figure-1: Immunofluorescent analysis of a rat cerebellum section stained with chicken pAb to a-internexin, (37-1054), dilution 1:5,000 in red, and costained with mouse mAb to MBP, 34-1072, 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of the rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. The a-internexin antibody selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells, parallel fibers and the axons of granule cells, while the MBP antibody stains myelin sheathes around axons.
34-1054 Image
Figure-2: Western blot analysis of different tissue lysates using chicken pAb to a-Internexin, (34-1054), dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] cow spinal cord and [7] pig spinal cord. 34-1054 antibody reveals the a-internexin protein with apparent molecular weight of 64 to 66kDa, with some variability among different species.
| Manufacturer: | Abeomics |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | 66 kDa neurofilament protein||Neurofilament 5 |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ul, 50 ul |
| Host name: | Chicken |
| Clone: | |
| Isotype: | IgY |
| Immunogen: | Purified recombinant rat Alpha -internexin expressed in and purified from E. coli. |
| Application: | ICC, IF, WB, IHC |
Additional Text
Gene ID
9118
Gene Name
INA
Uniprot ID
Q16352
Antibody Clonality
Polyclonal
Application Notes
Western blot: 1:10,000. ICC/IF and IHC: 1:1,000.
Storage Note
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
34-1054 Datasheet
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