Polyclonal Antibody to a-internexin/NF66
Catalogue Number: 34-1055-ABO
34-1055 Image
34-1055 Image
34-1055 Image
Figure-1: Immunofluorescent analysis of rat cerebellum section stained with rabbit pAb to a-internexin,(34-1055), dilution 1:2,000, in green, and chicken pAb to GFAP,(34-1046), dilution 1:5,000, in red. Blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with above antibodies. The a-internexin antibody selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells and parallel fibers the axons of granule cells. The GFAP antibody labels network of glial cells, such as astrocytes in the granule cell layer and white matter and Bergmann glia in the molecular layer.
34-1055 Image
Figure-2: Western blot analysis of whole tissue lysates using rabbit pAb to a-internexin,(34-1055), dilution 1:10,000 in green: [1] protein standard (red), [2] mouse spinal cord, [3] rat spinal cord, [4] bovine spinal cord. Major bands in the 64-66kDa range corresponds to a-internexin. The a-internexin protein from different species is known to vary slightly in SDS-PAGE molecular weight.
| Manufacturer: | Abeomics |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | 66 kDa neurofilament protein||Neurofilament 5 |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ul, 50 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Purified recombinant rat Alpha -internexin expressed in and purified from E. coli. |
| Application: | ICC, IF, WB, IHC |
Additional Text
Gene ID
9118
Gene Name
INA
Uniprot ID
Q16352
Antibody Clonality
Polyclonal
Application Notes
Western blot: 1:10,000-1:20,000. ICC/IF and IHC: 1:500-1:1,000.
Storage Note
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
34-1055 Datasheet
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