Polyclonal Antibody to Lamin A/C
Catalogue Number: 34-1057-ABO
34-1057 Image
34-1057 Image
34-1057 Image
Figure-1: Immunofluorescent analysis of HeLa cells stained with chicken pAb to lamin A/C,(34-1057), dilution 1:2,000 in red, and costained with mouse mAb to actin,(34-1002), dilution 1:500, in green. The blue is Hoechst staining of nuclear DNA. The (34-1057) antibody specifically labels the nuclear lamina, while the actin antibody stains the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites.
34-1057 Image
Figure-2: Western blot analysis of cytosolic or nuclear enriched fractions of cell lines probed with chicken pAb to lamin A/C,(34-1057), dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa cytosol, [3] HeLa nuclear, [4] NIH-3T3 cytosol, and [5] NIH-3T3 nuclear fractions. Two strong bands at 65kDa and 74kDa correspond to lamin A and lamin C proteins respectively, detected exclusively in the nuclear fractions. The same blot was simultaneously probed with mouse mAb to GAPDH, (34-1047), in red. The single band at 37kDa represents GAPDH protein which is expressed predominantly in the cytosolic fractions.
| Manufacturer: | Abeomics |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | 70 kDa lamin, Renal carcinoma antigen NY-REN-32, LMN1 |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ul, 50 ul |
| Host name: | Chicken |
| Clone: | |
| Isotype: | IgY |
| Immunogen: | Full length human Lamin A purified from E. coli. |
| Application: | ICC, IF, WB |
Additional Text
Gene Name
LMNA
Gene ID
4000
Uniprot ID
P02545
Antibody Clonality
Polyclonal
Application Notes
WB: 1:2,000. IF/ICC 1:1,000
Storage Note
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
34-1057 Datasheet
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