Figure-1: Immunofluorescent analysis of HeLa cells stained with chicken pAb to lamin A/C,(34-1057), dilution 1:2,000 in red, and costained with mouse mAb to actin,(34-1002), dilution 1:500, in green. The blue is Hoechst staining of nuclear DNA. The (34-1057) antibody specifically labels the nuclear lamina, while the actin antibody stains the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites.
34-1057 Image
Figure-2: Western blot analysis of cytosolic or nuclear enriched fractions of cell lines probed with chicken pAb to lamin A/C,(34-1057), dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa cytosol, [3] HeLa nuclear, [4] NIH-3T3 cytosol, and [5] NIH-3T3 nuclear fractions. Two strong bands at 65kDa and 74kDa correspond to lamin A and lamin C proteins respectively, detected exclusively in the nuclear fractions. The same blot was simultaneously probed with mouse mAb to GAPDH, (34-1047), in red. The single band at 37kDa represents GAPDH protein which is expressed predominantly in the cytosolic fractions.