Monoclonal Antibody to Lamin A/C (Clone: 4C4)
Catalogue Number: 34-1058-ABO
34-1058 Image
34-1058 Image
34-1058 Image
Figure-1: Immunofluorescent analysis of HeLa cells stained with mouse mAb to lamin A/C,(34-1058), dilution 1:2,000 in red, and costained with rabbit pAb to HSP60, dilution 1:5,000, in green. The blue is Hoechst staining of nuclear DNA. (34-1058) antibody specifically labels the nuclear lamina, while the rabbit pAb to HSP60 antibody reveals protein expressed in mitochondria.
34-1058 Image
Figure-2: Western blot analysis of different cell lysates using mouse mAb to lamin A/C,(34-1058), dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa, [3] HEK293 [4] C6, and [5] NIH-3T3 cell lysates. Two strong bands at 74 and 65kDa correspond to the lamin A and lamin C proteins respectively, detected only in the cells of human origin. (34-1058) antibody failed to recognize rat or mouse proteins.
| Manufacturer: | Abeomics |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | 70 kDa lamin, Renal carcinoma antigen NY-REN-32, LMN1 |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ul, 50 ul |
| Host name: | Mouse |
| Clone: | 4C4 |
| Isotype: | IgG1 |
| Immunogen: | Full length human Lamin A purified form E. coli. |
| Application: | ICC, IF, WB |
Additional Text
Gene Name
LMNA
Gene ID
4000
Uniprot ID
P02545
Antibody Clonality
Monoclonal
Application Notes
WB: 1:1,000-1:2,000. IF/ICC 1:1,000
Storage Note
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
34-1058 Datasheet
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