Figure-1: Immunofluorescent analysis of HeLa cells stained with mouse mAb to lamin A/C,(34-1058), dilution 1:2,000 in red, and costained with rabbit pAb to HSP60, dilution 1:5,000, in green. The blue is Hoechst staining of nuclear DNA. (34-1058) antibody specifically labels the nuclear lamina, while the rabbit pAb to HSP60 antibody reveals protein expressed in mitochondria.
34-1058 Image
Figure-2: Western blot analysis of different cell lysates using mouse mAb to lamin A/C,(34-1058), dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa, [3] HEK293 [4] C6, and [5] NIH-3T3 cell lysates. Two strong bands at 74 and 65kDa correspond to the lamin A and lamin C proteins respectively, detected only in the cells of human origin. (34-1058) antibody failed to recognize rat or mouse proteins.