Monoclonal Antibody to Beta Tubulin(Clone: 4E4)
Catalogue Number: 34-1117-ABO
34-1117 Image
34-1117 Image
34-1117 Image
Figure-1: Immunofluorescence analysis of HeLa cells stained with mouse mAb to β-tubulin,(34-1117), dilution 1:5,000 in green, and costained with chicken pAb to lamin A/C,(34-1057), dilution 1:2,000, in red. Blue is DAPI staining of nuclear DNA. (34-1117) antibody produces strong staining of cytoplasmic microtubules, while the lamin A/C antibody specifically labels the nuclear membrane of these cells.
34-1117 Image
Figure-2: Western blot analysis of equal amounts of cell line and whole brain lysates using mouse mAb to β-tubulin (34-1117), dilution 1:5,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS-1, [6] NIH-3T3, [7] C6 cells, [8] rat brain, and [9] mouse brain. Strong band at 50 kDa corresponds to the β-tubulin proteins.
| Manufacturer: | Abeomics |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Tubulin beta-5 chain |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ul, 50 ul |
| Host name: | Mouse |
| Clone: | 4E4 |
| Isotype: | IgG2a |
| Immunogen: | Pig brain tubulin |
| Application: | ICC, IF, WB, IHC |
Additional Text
Gene Name
TUBB
Uniprot ID
P07437
Gene ID
203068
Antibody Clonality
Monoclonal
Application Notes
WB: 1:5,000-1:10,000, ICC/IF and IHC: 1:1000-1:5,000.
Storage Note
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
34-1117 Datasheet
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